This Mouse Interferon Alpha/Beta Receptor 1 ELISA Kit from Innovative Research is intended for quantitative detection of mouse IFNAR1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse IFNAR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IFNAR1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E27-T429) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IFNAR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
- Detection Target: Interferon Alpha/Beta Receptor 1
- Uniprot ID: )
- Reactivity: Mouse
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 62.5pg/ml-4000pg/ml
- Sensitivity: <10pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Interferon-alpha/beta receptor alpha chain is a protein that in humans is encoded by the IFNAR1 gene. It is mapped to 16 C3.3; 16 52.98 cM. The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor.; Immunogen sequence: 369
