Mouse Insulin Like Growth Factor 1 Receptor ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Insulin Like Growth Factor 1 Receptor in biofluid samples, such as tissue homogenates, cell lysates or other biological fluids.This is a sandwich ELISA in strip well format with reagents for up to 96 tests. IGF1R present in samples reacts with an anti-Mouse IGF1R capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-IGF1R detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active IGF1R in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse IGF1R. Detection Target: Mouse Insulin Like Growth Factor 1 Receptor Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.068ng/mL Storage Conditions: Store all components at 4┬░C This Mouse IGF1R activity assay is for the quantitative determination of Insulin Like Growth Factor 1 Receptor in samples including tissue homogenates, cell lysates or other biological fluid For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.